BRAF Gene Mutation

BRAF Gene Mutation Detection Kit (PCR-fluorescence probe method).

Detection index: Three (3) mutations of BRAF gene.

Intended use: V600E, V600D, V600K mutations in exon 15 of the BRAF gene for qualitative detection of melanoma, colorectal cancer or lung cancer patients.

Sample type: Paraffin-embedded tissue, fresh tissue, frozen tissue sections, plasma, etc.

Packing specifications: Single tube, 24 tests/box.

Storage conditions and expiration date: Store at -20±5°C, avoid repeated freezing and thawing; the validity period is twelve (12) months from the production date indicated on the box.

Transportation: Use dry ice (or blue ice) to keep cool. The detection performance of this kit is not affected within 72 hours. The detection performance is not affected within 8 hours after opening (thawing).

Product advantages:

- Simple and fast: Single-tube single-serving packaging does not need reagent preparation process, only need to add samples. With FFPE tissue rapid reagent, rapid extraction is achieved in 15 minutes, and the entire experimental process is less than 1.5 hours.

- Direct rapid PCR reaction: A new generation of FAST-Taq DNA polymerase with hot start, rapid amplification and anti-inhibitory properties can amplify the sample to ensure sensitivity and specificity; dUTP+UDG enzyme anti-pollution system is added to the amplification system to avoid contamination. Internal standard quality control, monitoring sample extraction, amplification, to avoid false positives.

- Full color-tracking system: The extracted sample solution contains a blue tracer, which can indicate whether the template is added or not, and reduces the sampling error.

- High sensitivity: It can detect 10ng-100ng wild-type human genomic DNA and detect samples containing 1% mutation.